Does grape seed extract potentiate the inhibition of platelet reactivity in the presence of endothelial cells?

作者：Luzak Boguslawa^*; Kosiorek Anna; Syska Kamila; Rozalski Marek; Bijak Michal; Podsedek Anna; Balcerczak Ewa; Watala Cezary; Golanski Jacek

来源：Advances in Medical Sciences, 2014, 59(2): 178-182.

DOI：10.1016/j.advms.2014.02.005

• Grape seed extract
• Endothelial cells
• Platelet reactivity
• Cardiovascular disease

Purpose: Numerous studies have suggested that grape seed extract (GSE) confers vascular protection due to the direct effect of its polyphenol content on endothelial cells. The aim of the study was to determine whether GSE confers vascular protection through the direct effect of its polyphenol content on endothelial cells.
Material/ methods:After incubation with GSE-treated human umbilical vein endothelial cells (HUVECs), blood platelet reactivity was evaluated with regard to the expression of CD62P and the activated form of GPIIbIIIa in ADP-stimulated platelets.
Results: Lower concentrations of GSE were found to enhance the antiplatelet action of HUVECs: 1 mu g/ ml GSE reduced platelet reactivity by about 10%. While platelet reactivity was not altered by HUVECs incubated with higher concentrations of GSE, HUVEC proliferation was significantly reduced by GSE of up to 10 mu g gallic acid equivalent/ ml.
Conclusions: The results of the study show that low doses of GSE potentiate the inhibitory action of HUVECs on platelet reactivity, which may account, at least partially, for the protective effects of grape products against cardiovascular diseases. In contrast, high concentrations of GSE significantly impair endothelial cell proliferation in vitro.

• 出版日期2014-9