A new disruption vector (pDHO) to obtain heterothallic strains from both Saccharomyces cerevisiae and Saccharomyces pastorianus

作者：Blasco Lucia; Veiga Crespo Patricia; Vinas Miguel; Villa Tomas G^*

来源：International Microbiology, 2011, 14(4): 201-206.

DOI：10.2436/20.1501.01.149

• Saccharomyces cerevisiae
• Saccharomyces pastorianus
• homothallic
• heterothallic
• gene deletion

Yeasts are responsible for several traits in fermented beverages, including wine and beer, and their genetic manipulation is often necessary to improve the quality of the fermentation product. Improvement of wild-type strains of Saccharomyces cerevisiae and Saccharomyces pastorianus is difficult due to their homothallic character and variable ploidy level. Homothallism is determined by the HO gene in S. cerevisiae and the Sc-HO gene in S. pastorianus. In this work, we describe the construction of an HO disruption vector (pDHO) containing an HO disruption cassette and discuss its use in generating heterothallic yeast strains from homothallic Saccharomyces species. [Int Microbiol 2011; 14(4):201-206]

• 出版日期2011-12