Acidic preconditioning protects against ischemia-induced brain injury

作者：Zhang, Chen-hui; Fan, Yan-ying; Wang, Xiao-fen; Xiong, Jia-yan; Tang, Ying-ying; Gao, Jie-qiong; Shen, Zhe; Song, Xiao-hui; Zhang, Jing-ying; Shen, Yao; Li, Qing; Zhang, Xiangnan; Chen, Zhong^*

来源：Neuroscience Letters, 2012, 523(1): 3-8.

DOI：10.1016/j.neulet.2012.05.015

• Cerebral ischemia
• Preconditioning
• Acidic preconditioning
• Apoptosis

Ischemic preconditioning protects against cerebral ischemia. Recent investigations indicated that acidic preconditioning (APC) protects against ischemia-induced cardiomyocytes injury. However, it is not clear whether APC can protect against cerebral ischemia. To address this issue. C57BL/6 mice were exposed 3 times at 10-min intervals to a normoxic atmosphere containing 20% CO2 for 5 min before being further subjected to bilateral common carotid artery occlusion. APC reversed the ischemia-induced brain injury as revealed by improved performance in passive avoidance experiments and decreased neuron loss in the hippocampal CA1 region. Consistently, both APC-treated brain slices and primary cultured neurons were more resistant to oxygen-glucose-deprivation (OGD)-induced injury, in a pH- and time-dependent manner, as revealed by reversed cell/tissue viability. In addition, the APC treatment prevented OGD-induced mitochondrial transmembrane potential loss and apoptosis, which was inhibited by the mitochondrial permeability transport pore opener atractyloside. Taken together, these findings indicated that APC protects against ischemia-induced neuronal injury. The beneficial effects may be attributed, at least in part, to decreased mitochondria-dependent neuronal apoptosis.

• 出版日期2012-8-8
• 单位浙江大学; 温州医科大学; 山西医科大学