Molecular analysis of the gene encoding a cold-adapted halophilic subtilase from deep-sea psychrotolerant bacterium Pseudoalteromonas sp SM9913: cloning, expression, characterization and function analysis of the C-terminal PPC domains

作者：Yan Bing Qiang; Chen Xiu Lan; Hou Xiao Yan; He Hailun; Zhou Bai Cheng; Zhang Yu Zhong^*

来源：Extremophiles, 2009, 13(4): 725-733.

DOI：10.1007/s00792-009-0263-1

• MCP-03
• Subtilase
• Cold-adapted
• Halophilic
• Pseudoalteromonas sp SM9913
• PPC domain
• Deep sea

Only a few cold-adapted halophilic proteases have been reported. Here, the gene mcp03 encoding a cold-adapted halophilic protease MCP-03 was cloned from deep-sea psychrotolerant bacterium Pseudoalteromonas sp. SM9913, which contains a 2,130-bp ORF encoding a novel subtilase precursor. The recombinant MCP-03, expressed in Escherichia coli BL21 and purified from fermented broth, is a multi-domain protein with a catalytic domain and two PPC domains. Compared to mesophilic subtilisin Carlsberg, MCP-03 had characteristics of a typical cold-adapted enzyme (e.g., higher activity at low temperatures, lower optimum temperature and higher thermolability). MCP-03 also exhibited good halophilic ability with maximal activity at 3 M NaCl/KCl and good stability in 3 M NaCl. Deletion mutagenesis showed that the C-terminal PPC domains were unnecessary for enzyme secretion but had an inhibitory effect on MCP-03 catalytic efficiency and were essential for keeping MCP-03 thermostable.

• 出版日期2009-7
• 单位山东大学