Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf

作者：Shyaula Sajan L^*; Tamang Tensangmu; Ghouri Nida; Adhikari Achyut; Marasini Shanti; Bajracharya Gan B; Manandhar Mangala D; Choudhary Muhammad Iqbal

来源：Natural Product Research, 2016, 30(22): 2590-2593.

DOI：10.1080/14786419.2015.1114941

• Aconitum spicatum
• antileishmanial activity
• Leishmania major
• diterpenoid alkaloids

The crude extracts of tubers of Aconitum spicatum (Bruhl) Stapf were investigated for in vitro antileishmanial activity against Leishmania major. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC50 value of 27.10 +/- 0.0g/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (1), chasmaconitine (2) and ludaconitine (3). Compounds 3 and 2 showed antileishmanial activity with IC50=36.10 +/- 3.4 and 56.30 +/- 2.1g/mL, respectively. Compound 1 was less effective (IC50>100g/mL). The cytotoxicity of compounds 1, 2 and 3 studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30M.

• 出版日期2016