Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands

作者：Li, Haihong^*; Chen, Liyun; Zhang, Mingjun; Xie, Sitian; Cheng, Liuhanghang

来源：Acta Histochemica, 2018, 120(6): 520-524.

DOI：10.1016/j.acthis.2018.06.003

• Eccrine sweat glands
• Three-dimensional reconstruction
• FoxA1
• GCDFP-15
• Na+-K+-ATPase alpha
• NKCC1

Previously studies showed that Forkhead transcription factor A1 (FoxA1) was associated with sweat secretion. To investigate the expression and localization of FoxA1 in the three-dimensional (3D) reconstructed eccrine sweat glands, eccrine sweat gland cells were transplanted subcutaneously into nude mice with Matrigel, and at 2, 3, 4, 5, 6, 8, 10 and 12 weeks post-transplantation, the reconstructed eccrine sweat glands were removed and immunostained for FoxA1 and co-immunostained for FoxA1 and eccrine sweat markers, K7, carbonic anhydrase II (CA II), gross cystic disease fluid protein-15 (GCDFP-15) and alpha-smooth muscle actin (alpha-SMA), and FoxA1 and sweat secretion-related proteins, Na+-K+-ATPase alpha and Na+-K+-2Cl- cotransporter 1 (NKCC1). The results showed that FoxA1-positive cells weren't detected until 3 weeks post-implantation, a time point of the differntiation of secretory coil-like structures. From the fourth week on, the number of FoxA1-positive cells increased and thereafter maintained at a high number. Double immunofluorescence staining showed that FoxA1-positive cells co-expressed dark cell marker GCDFP-15 and myoepithelial cell marker alpha-SMA, as well as secretion-related proteins, Na+-K+-ATPase alpha and NKCC1 in both the native and reconstructed eccrine sweat glands. In conclusion, FoxA1 might be related to the development and differentiation of secretory coil-like structures, as well as the secretory function of the 3D reconstructed eccrine sweat glands.

• 出版日期2018
• 单位汕头大学