A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency

作者：Shen, J.; Zhao, D. J.; Li, W.; Hu, Q. L.; Wang, Q. W.; Xu, F. J.; Tang, G. P.^*

来源：Biomaterials, 2013, 34(18): 4520-4531.

DOI：10.1016/j.biomaterials.2013.02.068

• Gene delivery
• Vector
• Polyethylenimine
• Biodegradable
• Mimic

The low toxicity and efficient gene delivery of polymeric vectors remain the major barrier to the clinical application of non-viral gene therapy. Here, we present a poly-D, c-succinimide (PSI)-based biodegradable cationic polymer which mimicked the golden standard, branched polyethylenimine (PEI, similar to 25 kDa). To investigate the influence of 1 degrees, 2 degrees, 3 degrees amine group ratio in the polymer, a series of PSI-based vectors (PSI-NNx'-NNy) grafted with different amine side chains of N,N-dimethyldipropylenetriamine (NN') and bis(3-aminopropyl)amine (NN) were first characterized and contrasted by biophysical measurements. The in vitro and in vivo biological assay demonstrated that PSI-NN0.85'-NN1 exhibited better transfection ability and biocompatibility than PEI. The present results suggest that such PEI-mimic biodegradable PSI-NN0.85'-NN1 possesses a good potential application for clinical gene delivery.

• 出版日期2013-6
• 单位北京化工大学