Neonatal exposure to BDE 209 impaired learning and memory, decreased expression of hippocampal core SNAREs and synaptophysin in adult rats

作者：Li, Xiong; Wang, Chunmei; Wang, Wen; Yue, Chengwei; Tang, Yan^*

来源：NeuroToxicology, 2017, 59: 40-48.

DOI：10.1016/j.neuro.2017.01.004

• Decabromodiphenyl ether
• Hippocampus
• SNARES
• Synaptophysin
• Developmental neurotoxicity
• Learning and memory

Polybrominated diphenyl ethers (PBDEs) are a class of flame retardants. While the mechanism remains unknown, the potential neurotoxic effects of PBDEs remain a relevant issue. In the present study, neonatal Sprague-Dawley rats of both sexes were administered BDE 209 (1,10, or 20 mg/kg body weight) or peanut oil once daily from postnatal day (PND) 5 to PND 10. We examined the spatial learning and memory by Morris water maze and the working and reference memory by eight-arm radial maze in the stage of adulthood. Compared with controls, significantly longer escape latencies and fewer platform crossings in the Morris water maze were observed in rats exposed to 1,10, and 20 mg/kg BDE 209, and these effects were dose-dependent. Significantly higher working and reference memory error rates in the eight-arm radial maze were also observed in rats exposed to 10 and 20 mg/kg BDE 209. Furthermore, we detected the mRNA and protein expressions of hippocampal synaptobrevin 2, syntaxin 1A, Synaptosome Associated Protein 25 (SNAP-25), and synaptophysin using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot methods. Compared with controls, the mRNA expressions of synaptobrevin 2, syntaxin 1A, SNAP-25, and synaptophysin were significantly decreased in the hippocampi of rats exposed to 1, 10, and 20 mg/kg BDE 209, and the protein expressions of synaptobrevin 2 and SNAP-25 were significantly decreased in the hippocampi of rats exposed to 10 and 20 mg/kg BDE 209, while syntaxin 1A and synaptophysin were significantly decreased in rats exposed to 1, 10, and 20 mg/kg BDE 209. Alterations that may be involved in the learning and memory deficits induced by BDE 209 reveal the possibility of synapse loss.

• 出版日期2017-3
• 单位西南医科大学