Negative regulation of peroxiredoxin 6 (Prdx 6) transcription by nuclear oncoprotein DEK during leukemia cell differentiation

作者：Kim Kee Beom; Chae Yun Cheol; Han Arim; Kang Joo Young; Jung Hyeonsoo; Park Jin Woo; Hahm Ja Young; Kim Seryeon; Seo Sang Beom^*

来源：Animal Cells and Systems, 2014, 18(5): 318-323.

DOI：10.1080/19768354.2014.950605

• Prdx 6
• DEK
• leukemia
• differentiation

The oncogene protein DEK is an abundant and ubiquitous nuclear protein with implications in acute myelogenous leukemia, as translocation which results in the formation of a DEK-CAN fusion protein. In a previous study, we have identified that DEK negatively regulated peroxiredoxin 6 (Prdx 6) transcription synergistically with the p65 subunit of NF-kappa B. In this study, we further investigated DEK-mediated transcriptional regulation of Prdx 6 during leukemia cell differentiation. Using Chromatin Immunoprecipitation analysis and Prdx 6 reporter assays, we found that DEK operated as a negative regulator of Prdx 6 transcription during leukemia cell differentiation. DEK was highly expressed and recruited to Prdx 6 promoter along with p65 and repressed transcription after leukemia cell differentiation.

• 出版日期2014-9-3