科研之友
微信
新浪微博
Facebook
分享链接
摘要
The present study investigated the effect of fungal pretreatment on cotton gin waste by solid and submerged state of cultivation and screening potential fungus for delignification. Cotton gin waste was treated with white rot fungi namely, Trametes pubscens, Pleurotus ostreatus, Pycnoporus cinnabarinus and Phanerochaete chrysosporium to separate cellulose and hemicellulose components by degrading lignin from a complex mixture of the above three components using their secreted enzymes. In the delignification process, solid state cultivation (SSC) was found to be more effective than submerged cultivation (SMC). Among the four fungi used in the study, Pycnoporus cinnabarinus showed better result in achieving lignin removal of 55.2 and 40.2% in solid and submerged cultivation respectively. The corresponding cellulose and hemicellulose reduction was determined as 61.9 and 70% in SSC, whereas their value in SMC was 44% cellulose and 56.3% hemicellulose. The confirmation of delignification process with respect to fungal pretreated and untreated cotton gin waste was assessed using FTIR, XRD and SEM analysis. Optimization of parameters for Pycnoporus cinnabarinus further showed substantial improvement in lignin removal i.e., 60% in SSC at pH, shaking speed and temperature of 4.5, 138 rpm and 32 degrees C respectively.
