Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case

作者：Lee S; Kim J; Son JS; Nam J; Jeong DH; Lee K; Jang S; Yoo J; Lee J; Lee DY; Kang HG; An GH^*

来源：Plant and Cell Physiology, 2003, 44(12): 1403-1411.

DOI：10.1093/pcp/pcg156

• insertional mutagenesis
• MADS-box genes
• pool screening
• rice
• T-DNA

We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to [e-mail].

• 出版日期2003-12