Engineering beta-lactamase zymogens for use in protease activity assays

作者：Kim Hajin; Yoon Hyun Kyung; Yoo Tae Hyeon^*

来源：Chemical Communications, 2014, 50(70): 10155-10157.

DOI：10.1039/c4cc04549a

In this study, the concept of autoinhibition, a mechanism of protein activity regulation, was applied to the design and engineering of a beta-lactamase zymogen. Using this zymogen, a sensitive protease assay method was developed in which activation of the zymogen by proteases produces an amplified absorbance signal. The approach reported here can be adapted for engineering of zymogens as biological sensors and components of synthetic signaling pathways.

• 出版日期2014