A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation

作者：Klykov S P^*; Kurakov V V; Vilkov V B; Demidyuk I V; Gromova T Yu; Skladnev D A

来源：Biofabrication, 2011, 3(4): 045006.

DOI：10.1088/1758-5082/3/4/045006

In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis(6)] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.

• 出版日期2011-12