Accurate and Highly Sensitive Screening of DNA-binding Molecules with Primer-dimer Templates Using a Real-time PCR Analyzer

作者：Inoue Akira; Kumeda Tsunehiro; Nakamura Hiroshi^*

来源：Analytical Sciences, 2011, 27(5): 461-463.

DOI：10.2116/analsci.27.461

We have developed a strategy to detect the DNA-binding affinities of chemical compounds with a real-time PCR analyzer. In this system, the DNA-binding affinities of chemical compounds are efficiency detected by PCR with high sensitivity. To obtain accurate information about the DNA-binding affinities of chemical compounds, primer-dimers prepared in advance are used as templates. The denature temperature is settled to around the melting temperature of the templates.

• 出版日期2011-5