Age-Dependent Defects of Regulatory B Cells in Wiskott-Aldrich Syndrome Gene Knockout Mice

作者：Yokoyama Tadafumi; Yoshizaki Ayumi; Simon Karen L; Kirby Martha R; Anderson Stacie M; Candotti Fabio^*

来源：PLos One, 2015, 10(10): e0139729.

DOI：10.1371/journal.pone.0139729

The Wiskott-Aldrich syndrome (WAS) is a rare X-linked primary immunodeficiency characterized by recurrent infections, thrombocytopenia, eczema, and high incidence of malignancy and autoimmunity. The cellular mechanisms underlying autoimmune complications in WAS have been extensively studied; however, they remain incompletely defined. We investigated the characteristics of IL-10-producing CD19(+)CD1d(high)CD5(+) B cells (CD1d(high)CD5(+) Breg) obtained from Was gene knockout (WKO) mice and found that their numbers were significantly lower in these mice compared to wild type (WT) controls. Moreover, we found a significant age-dependent reduction of the percentage of IL-10-expressing cells in WKO CD1d(high)CD5(+) Breg cells as compared to age-matched WT control mice. CD1d(high)CD5(+) Breg cells from older WKO mice did not suppress the in vitro production of inflammatory cytokines from activated CD4(+) T cells. Interestingly, CD1d(high)CD5(+) Breg cells from older WKO mice displayed a basal activated phenotype which may prevent normal cellular responses, among which is the expression of IL-10. These defects may contribute to the susceptibility to autoimmunity with age in patients with WAS.

• 出版日期2015-10-8
• 单位NIH