Novel metastatic models of esophageal adenocarcinoma derived from FLO-1 cells highlight the importance of E-cadherin in cancer metastasis

作者：Liu David S; Hoefnagel Sanne J M; Fisher Oliver M; Krishnadath Kausilia K; Montgomery Karen G; Busuttil Rita A; Colebatch Andrew J; Read Matthew; Duong Cuong P; Phillips Wayne A^*; Clemons Nicholas J^*

来源：Oncotarget, 2016, 7(50): 83342-83358.

DOI：10.18632/oncotarget.13391

• metastasis
• esophageal cancer
• CDH1
• E-cadherin
• animal models

There is currently a paucity of preclinical models available to study the metastatic process in esophageal cancer. Here we report FLO-1, and its isogenic derivative FLO-1(LM), as two spontaneously metastatic cell line models of human esophageal adenocarcinoma. We show that FLO-1 has undergone epithelial-mesenchymal transition and metastasizes following subcutaneous injection in mice. FLO-1(LM), derived from a FLO-1 liver metastasis, has markedly enhanced proliferative, clonogenic, antiapoptotic, invasive, immune-tolerant and metastatic potential. Genome-wide RNAseq profiling revealed a significant enrichment of metastasis-related pathways in FLO-1(LM) cells. Moreover, CDH1, which encodes the adhesion molecule E-cadherin, was the most significantly downregulated gene in FLO-1(LM) compared to FLO-1. Consistent with this, repression of E-cadherin expression in FLO-1 cells resulted in increased metastatic activity. Importantly, reduced E-cadherin expression is commonly reported in esophageal adenocarcinoma and independently predicts poor patient survival. Collectively, these findings highlight the biological importance of E-cadherin activity in the pathogenesis of metastatic esophageal adenocarcinoma and validate the utility of FLO-1 parental and FLO-1(LM) cells as preclinical models of metastasis in this disease.

• 出版日期2016-12-13