A rare point mutation in the Ras oncogene in hepatocellular carcinoma

作者：Taketomi Akinobu^*; Shirabe Ken; Muto Jun; Yoshiya Shohei; Motomura Takashi; Mano Yohei; Ikegami Tohru; Yoshizumi Tomoharu; Sugio Kenji; Maehara Yoshihiko

来源：Surgery Today, 2013, 43(3): 289-292.

DOI：10.1007/s00595-012-0462-8

• Ras
• Mutation
• Hepatocellular carcinoma
• Sorafenib

The Ras gene is one of the oncogenes most frequently detected in human cancers, and codes for three proteins (K-, N-, and H-Ras). The aim of this study was to examine the mutations in codons 12, 13 and 61 of the three Ras genes in cases of human hepatocellular carcinoma (HCC). %26lt;br%26gt;Paired samples of HCC and corresponding non-malignant liver tissue were collected from 61 patients who underwent hepatectomy. A dot-blot analysis was used to analyze the products of the polymerase chain reaction (PCR) amplification of codons 12, 13, and 61 of K-, N- and H-Ras for mutations. %26lt;br%26gt;Only one mutation (K-Ras codon 13; Gly to Asp) was detected among the 61 patients. Interestingly, this patient had a medical history of surgery for both gastric cancer and right lung cancer. No mutations were found in codons 12 and 61 of K-Ras or codons 12, 13 and 61 of the N-Ras and H-Ras genes in any of the HCCs or corresponding non-malignant tissues. %26lt;br%26gt;These findings indicated that the activation of Ras proto-oncogenes by mutations in codons 12, 13, and 61 does not play a major role in hepatocellular carcinogenesis.

• 出版日期2013-3