Nitrogen metabolism in actinorhizal nodules of Alnus glutinosa: Expression of glutamine synthetase and acetylornithine transaminase

作者：Guan CG; Ribeiro A; Akkermans ADL; Jing YX; vanKammen A; Bisseling T; Pawloski K

来源：Plant Molecular Biology, 1996, 32(6): 1177-1184.

DOI：10.1007/BF00041403

• actinorhiza
• nodules
• Alnus glutinosa
• symbiotic nitrogen fixation
• nitrogen metabolism
• glutamine synthetase
• acetylornithine transaminase
• in situ hybridization

Two nodule cDNA clones representing genes involved in Alnus glutinosa nitrogen metabolism were analysed. ag11 encoded glutamine synthetase (GS), the enzyme responsible for ammonium assimilation, while ag118 encoded acetylornithine transaminase (AOTA), an enzyme involved in the biosynthesis of citrulline, the nitrogen transport form in Alnus. GS mRNA was found at highest levels in root nodules, where it was present in the infected cells as well as in the cells of the pericycle of the vascular system. AOTA transcripts were found at high levels in nodules, confined to the infected cells, suggesting that in nodules of A. glutinosa, citrulline biosynthesis takes place mainly in the infected cells.

• 出版日期1996-12
• 单位中国科学院植物研究所; 中国农业大学