科研之友
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摘要
This study established a new rat model of 1/4 partial nephrectomy for research on ischemia-reperfusion injury in residual kidney. Thirty healthy Sprague-Dawley rats were intraperitoneally anesthetized by pentobarbital sodium. A lumbar oblique incision was made, and then the right renal pedicle was isolated and clamped by a smooth microclip. The kidney size was measured and the upper pole of the right kidney (about 0.5 x 1.0 cm) was resected by a scorching knife and then SURGICEL* FIBRILLAR Absorbable Hemostats (oxidized regenerated cellulose) was used for rapid haemostasis. Mean kidney size was 2.0 x 1.1 cm, and mean kidney tissue removed actually was 0.6 x 1.0 cm. Mean operation time was 3.2 +/- 0.5 min (2.6-5.2 min), and mean haemostasis time after resection was 1.2 min (0.8-1.5 min). Three minutes after partial nephrectomy, the smooth microclip was removed for reperfusion, without rebleeding in all cases. No rats died during the operation. A new 1/4 partial nephrectomy rat model was successfully established in our study, which can be used for research on ischemia-reperfusion injury in residual kidney. Rapid haemostasis could be achieved by SURGICEL* FIBRILLAR Absorbable Hemostats, which is suitable for application in surgical experiments in rats.
- 出版日期2010-7-4
- 单位同济大学
