High-throughput FTIR spectroscopy of intact HepG2 cells reveals additive and non-additive effects of individual fatty acids when given as mixtures

作者：Najbjerg Heidi; Young Jette F^*; Bertram Hanne C; Afseth Nils Kristian; Host Vibeke; Kohler Achim

来源：Journal of Biophotonics, 2013, 6(5): 446-456.

DOI：10.1002/jbio.201200073

• infrared spectroscopy
• high throughput
• HepG2
• fatty acid mixture

In the present study we investigated the ability of high-throughput FTIR spectroscopy in combination with multivariate data analysis to reveal if any combinatory effects of fatty acids in mixture are present in liver HepG2 cell cultures after three days of exposure. For this investigation we used an experimental mixture design containing three different octadecenoic acids (oleic acid: C18:1 cis- 9, elaidic acid: C18:1 trans- 9 and vaccenic acid: C18:1 trans- 11) of a total concentration of 100 M. The results obtained revealed both additive and non-additive effects of individual fatty acids when combined in mixtures. Furthermore, we demonstrate by use of scanning electron microscopy that cells are preserved as intact structures ensuring that FTIR measurements are obtained on whole cell keeping cell compounds in their natural surroundings during measurements.

• 出版日期2013-5