Antiparallel RNA G-quadruplex Formed by Human Telomere RNA Containing 8-Bromoguanosine

作者:Xiao Chao Da; Ishizuka Takumi; Xu Yan
来源:Scientific Reports, 2017, 7(1): 6695.
DOI:10.1038/s41598-017-07050-w

摘要

In this study, by combining nuclear magnetic resonance (NMR), circular dichroism (CD), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), and gel electrophoresis, we report an unusual topological structure of the RNA G-quadruplex motif formed by human telomere RNA r(UAGGGU) containing 8-bromoguanosine. Results showed that the RNA sequence formed an antiparallel tetramolecular G-quadruplex, in which each pair of diagonal strands run in opposite directions. Furthermore, guanosines were observed both in syn- and anti-conformations. In addition, two of these G-quadruplex subunits were found to be stacking on top of each other, forming a dimeric RNA G-quadruplex. Our findings provide a new insight into the behavior of RNA G-quadruplex structures.

  • 出版日期2017-7-27