MEG3 regulates cell cycle progression via control of P53 expression in gastric cancer

作者：Yang, Xiaomin; Ren, Hao; Wei, Ran; Zhang, Xin; Zhang, Xiaoli; Wang, Chuanxin; Zhang, Yi^*

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(7): 6887-6895.

• Gastric cancer
• lncRNA-MEG3
• P53
• cell cycle
• therapeutic target

Gastric cancer is the second leading cause of cancer deaths worldwide. Recently lncRNA has been reported playing important role in many diseases especially in the progress of tumor formation. However the roles of many lncRNAs in gastric cancer remain largely unclear. LncRNA-MEG3 has been invested playing key role in many diseases especially in central nervous system diseases. In our study, we examined the expression of MEG3 and P53 mRNA in 59 gastric cancer tissues and their adjacent tissues. In addition we tested the expression level of lncRNA-MEG3 in gastric epithelial cells and gastric cancer cell lines such as AGS, BGC823, SGC7901, N87 using qRT-PCR and found that the expression level of MEG3 in gastric cancer tissues and cell lines were much lower than that of adjacent tissues and GES1 cell line. Transfection of pCDNA-MEG3 could significantly inhibit the cell cycle in BGC823 cell line. What's more, we also found the expression of protein P53 was upregulated in a great extent in the group of pCDNA-MEG3 compared with the. In conclusion our study suggested that lncRNA-MEG3 is down expression in gastric cancer and regulate the process of carcinogenesis and may serve as a therapeutic target of gastric cancer in future.

• 出版日期2016
• 单位山东大学