A Robust Graft-to Strategy To Form Multifunctional and Stealth Zwitterionic Polymer-Coated Mesoporous Silica Nanoparticles

作者：Zhu, Yongheng; Sundaram, Harihara S.; Liu, Sijun; Zhang, Lei; Xu, Xuewei; Yu, Qiuming; Xu, Jiaqiang^*; Jiang, Shaoyi

来源：Biomacromolecules, 2014, 15(5): 1845-1851.

DOI：10.1021/bm500209a

• DRUG-DELIVERY
• BIOLOGICAL APPLICATIONS
• SURFACE-CHEMISTRY
• ADSORPTION
• PROTEINS
• SYSTEM
• SBA-15
• FUNCTIONALIZATION
• SULFOBETAINE
• RELEASE

Mesoporous silica nanoparticles (MSNs) are a new class of carrier materials promising for drug/gene delivery and many other important applications. Stealth coatings are necessary to maintain their stability in complex media. Herein, a biomimetic polymer conjugate containing one ultralow fouling poly(carboxybetaine) (pCBMA) chain and one surface-adhesive catechol (DOPA) residue group was efficiently grafted to the outer surface of SBA-15 type MSNs using a convenient and robust method. The cytotoxicity of SBA-15-DOPA-pCBMAs was evaluated by the 344,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results showed no significant decrease in cell viability at the tested concentration range. Macrophage cell uptake studies revealed that the uptake ratios of SBA-15-DOPA-pCBMAs were much lower than that of parent MSNs. Furthermore, inductively coupled plasma mass spectrometry (ICP-MS) analysis results showed that after SBA-15-DOPA-pCBMAs were conjugated with a targeting cyclo-[Arg-Gly-Asp-o-Tyr-Lys] (cRGD) peptide, uptake by bovine aortic endothelial cells (BAECs) was notably increased. Results indicated that cRGD-functionalized MSNs were able to selectively interact with cells expressing alpha v beta 3 integrin. Thus, MSNs with DOPA-pCBMAs are promising as stealth multifunctional biocarriers for targeted drug delivery or diagnostics.

• 出版日期2014-5
• 单位上海第二工业大学; 上海大学