A Kunitz-type proteinase inhibitor from the midgut of the ixodid tick, Haemaphysalis longicornis, and its endogenous target serine proteinase

作者：Miyoshi Takeharu; Tsuji Naotoshi^*; Islam M Khyrul; Alim M Abdul; Hatta Takeshi; Yamaji Kayoko; Anisuzzaman; Fujisaki Kozo

来源：Molecular and Biochemical Parasitology, 2010, 170(2): 112-115.

DOI：10.1016/j.molbiopara.2009.12.005

• Tick
• Midgut
• Kunitz-type
• Inhibitor
• Serine proteinase

Although previous studies strongly suggested the involvement of serine proteases in blood digestion in the midgut of ticks, the regulating molecules of these proteinases are still unidentified. A novel Haemaphysalis longicornis Kunitz-type serine proteinase inhibitor with a single Kunitz-domain(HlMKI) has been identified and its co-localization with a midgut-derived serine proteinase (HISP) within the epithelial cells has been demonstrated. Recombinant HlMKI inhibited the hydrolytic activity of HISP, suggesting that HlMKI is a possible inhibitor of HISP and may be part of a regulatory system of midgut serine proteinases.

• 出版日期2010-4