A rapid and simple protocol for the transformation of plasmid DNA using CaCl2.

作者：Xiao GF; Qi YP; Li L; Yi W

来源：Progress in Biochemistry and Biophysics, 2000, 27(1): 96-98.

• plasmid
• transformation
• competent cells

A rapid and simple protocol for the transformation of plasmid DNA using CaCl2 is reported. 3 similar to 10 min incubation of cells with DNA on ice and spreading onto agar plates pre-warmed to 37 similar to degrees C, resulting in more than 10(5) colonies/mu g plasmid DNA. The effect of Ca2+ concentration and store time at 4 degrees C on transformation efficiency is also discussed.

• 出版日期2000-2
• 单位武汉大学