A chemically sulfated polysaccharide from Grifola frondos induces HepG2 cell apoptosis by notch1-NF-kappa B pathway

作者：Wang, Chun-ling^*; Meng, Meng; Liu, Sheng-bin; Wang, Li-rui; Hou, Li-hua; Cao, Xiao-hong

来源：Carbohydrate Polymers, 2013, 95(1): 282-287.

DOI：10.1016/j.carbpol.2013.02.057

• Grifola frondosa
• Sulfated polysaccharides
• Apoptosis
• Notch1
• NF-kappa B

Sulfated polysaccharides have been known to inhibit proliferation in tumor cells. However, the molecular mechanisms involved in sulfated polysaccharides-induced apoptosis are still uncharacterized. In this study, the effect of a chemically sulfated polysaccharide obtained from Grifola frondosa (S-GFB) on HepG2 cell proliferation and apoptosis-related mechanism were investigated. It was found that S-GFB inhibited proliferation of HepG2 cells in a dose-dependent manner with IC50 at 48 h of 61 mu g ml(-1). The results of scanning electron micrographs indicated that S-GFB induced typical apoptotic morphological feature in HepG2 cells. Flow cytometric analysis demonstrated that S-GFB caused apoptosis of HepG2 cells through cells arrested at S phase. Western-blotting results showed that S-GFB inhibited notch1 expression, I kappa B-alpha degradation and NF-kappa B/p65 translocation from cytoplasm into nucleus. Simultaneously, the apoptotic mechanism of HepG2 cells induced by S-GFB was associated with down regulation of FLIP, and activation of caspase-3 and caspase-8. Taken together, these findings suggest that the S-GFB induces apoptosis through a notch1/NF-kappa B/p65-mediated caspase pathway.

• 出版日期2013-5-5
• 单位天津科技大学