A lytic bacteriophage as a biocontrol for some enteropathogenic and enterohemorrhagic Escherichia coli strains of zoonotic risk in Egypt

作者：Askora A; Merwad A^*; Gharieb R M; Maysa A I A

来源：Revue de Medecine Veterinaire, 2015, 166(3-4): 76-83.

• Escherichia coli
• antibiotic resistance
• oZE1 phage
• siphoviridae
• biocontrol

This study investigated a lytic bacteriophage effect on growth of enteropathogenic and enterohemorrhagic Escherichia coli isolated from cattle and workers. Bacteriophage was isolated by single plaque isolation. Phage susceptibility was determined by spot test to forty four E. coli isolates. Electron micrograph and genome analysis of phage were achieved. Phage was inoculated into each culture of E. coli strains O26, O111 and O124 at multiplicity of infection of 1. The oZE1 phage was belonged to family Siphoviridae. Phage produced lysis on four E. coli strains. The means log(10) CFU/ml of E. coli O26, O111, O124 strains was significantly decreased in cultures infected with oZE1 phage than control. The oZE1 phage could be used as a biocontrol for enteropathogenic and enterohemorrhagic E. coli.

• 出版日期2015