A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantification of major phospholipids classes of donkey serum

作者：Seri Hisham I^*; Naim Hassan Y; Hassan Tigani

来源：Veterinarski Arhiv, 2010, 80(3): 365-373.

• donkeys
• ELSD
• HPLC
• phospholipd
• sphingolipid
• Sudan

An H PLC method with an evaporative laser scattering detector was used to quantify major phospholipids fractions in donkeys' serum. Blood samples were collected bimonthly for a whole year from 20 donkeys (10 male and 10 female) from the Sudanese breed kept at the premises of the Central Veterinary Research Laboratory (CVRL), Soba. The method used made the excellent separation possible of phosphatidylglycerine (PG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingomyelin (SM). in 27 minutes, including the regeneration of the column. The SM resulted in two peaks (saturated and unsaturated fatty acids), as described by other researchers. The method was unable to separate phosphatidylserine (PS), which appeared in one peak with phosphatidylinositol. There is a significant difference in the level of PG, PE, PI and SM between females and males. The method fully discussed in this study and the obtained values of major phospholipids can be used for health control and diagnosis of diseases.

• 出版日期2010-6