A "turn-on" fluorescence assay for lead(II) based on the suppression of the surface energy transfer between acridine orange and gold nanoparticles

作者：Wang, Xiao-Feng; Xiang, Li-Ping; Wang, Yong-Sheng^*; Xue, Jin-Hua; Zhu, Yu-Feng; Huang, Yan-Qin; Chen, Si-Han; Tang, Xian

来源：Microchimica Acta, 2016, 183(4): 1333-1339.

DOI：10.1007/s00604-015-1738-7

• Fluorescence enhancement
• Competitive binding
• Fluorescent probe
• Environmental analysis
• Fluorescence lifetime
• Zeta-potential

The authors describe a strategy for fluorometric determination of lead(II) that is based on the suppression of the surface energy transfer that occurs between acridine orange and gold nanoparticles (AuNPs). As a result, the fluorescence of the system is recovered. Under optimized conditions, the enhancement of fluorescence intensity is related to the concentration of lead(II) in the 44 nM to 4.8 mu M range, with a detection limit of 13 nM. The relative standard deviations for 11 determinations at concentrations of 0.386 mu M, 1.93 mu M and 2.89 mu M are 1.02 %, 1.06 % and 1.75 %, respectively. This result suggests that the method can potentially be used to monitor the level of lead(II) in environmental samples.

• 出版日期2016-4
• 单位南华大学