Embryogenesis and plant regeneration from anther culture in loquat (Eriobotrya japonica L.)

作者：Li Junqiang; Wang Yongqing^*; Lin Lihua; Zhou Lijun; Luo Nan; Deng Qunxian; Xian Junren; Hou Chunxia; Qiu Yuan

来源：Scientia Horticulturae, 2008, 115(4): 329-336.

DOI：10.1016/j.scienta.2007.10.007

• anther culture
• callus induction
• embryogenesis
• Eriobotrya japonica L.
• haploid
• plant regeneration

The object of this study was to induce embryogenesis and establish plant regeneration system for anther culture in loquat (Eriobotrya japonica L.). Cold pretreatment was a key factor, and supplement of 2,4-D in the media was absolutely necessary for induction of calluses from cultured loquat anthers. The best response of anthers to in vitro culture was obtained when a 48-h cold pretreatment was employed to flower buds at 4 degrees C in darkness. Genotype was a decisive factor for embryo differentiation. When anther-derived calluses of three loquat cultivars, i.e., cv. 'Longquan1', 'Dawuxing' and 'Zaozhong6', were transferred to embryo differentiation medium, embryos were induced only for cv. 'Dawuxing' on MS medium containing 3% sucrose, 0.23 mu M ZT in combination with 0.05 mu M NAA + 0.05 mu M IBA or 0.11 mu M NAA + 0.10 mu M IBA, and the differentiation rates were 3.33% and 10.00%, respectively. The results of histological studies showed that embryos developed through typical globular, heart, torpedo and cotyledon stages after 4 weeks of culture. The treatment designed to mature the embryos on medium containing 3% of sucrose at 4 degrees C under darkness for 4 weeks was effective for subsequent embryo germination and plant conversion, which gave rise to 72.5% plant recovery. Cytological studies showed that 26 plantlets were haploids (n = 17) and the remaining 4 plantlets were diploids for the 30 regenerants tested.

• 出版日期2008-2-21
• 单位四川农业大学