A novel porphyrin-based molecular probe ZnTCPPSpm4 with catalytic, stabilizing and chiroptical diagnostic power towards DNA B-Z transition

作者：Gangemi Chiara M A; D'Urso Alessandro; Tomaselli Gaetano A; Berova Nina^*; Purrello Roberto^*

来源：Journal of Inorganic Biochemistry, 2017, 173: 141-143.

DOI：10.1016/j.jinorgbio.2017.05.008

• Porphyrin derivative
• Z-DNA
• Circular dichroism
• B-Z transition
• Chiroptical probe
• Supramolecular chemistry

In this work we have designed a new zinc(II) porphyrin with four spermines conjugate in the meso positions, meso-tetrakis-(4-carboxysperminephenyl)porphyrin, ZnTCPPSpm4) with the aim of acting as a chiroptical probe for the Z-form of DNA, a high energy transient conformation of DNA. In addition to monitor by Electronic Circular Dichroism chiroptical response the formation of Z-DNA in the presence of micromolar concentration of spermine, this porphyrin based molecular probe is also able to catalyze and stabilize this important DNA structure. The ZnTCPPSpm4 conjugate represents a perfect example of single molecule, which possesses all these three properties at the same time. The increased stability of Z-DNA in the presence of this derivative opens possibility for further studies on the mechanism of B-to Z-DNA transition, and on the design of new probes with improved efficiency.

• 出版日期2017-8