Muscarinic acetylcholine receptors present in human osteoblast and bone tissue

作者：Liu, Pei-Shan^*; Chen, Yi-Yin; Feng, Chi-Kuang; Lin, Yi-Hsuan; Yu, Tien-Chi

来源：European Journal of Pharmacology, 2011, 650(1): 34-40.

DOI：10.1016/j.ejphar.2010.09.031

• Muscarinic acetylcholine receptor
• Human osteosarcoma HOS cell
• Human bone tissue
• Calcium signaling
• Proliferation

Acetylcholine is the predominant neurotransmitter in the neuromuscular Junction and a role in bone has been postulated The expression of nicotinic receptors has been reported in osteoblasts but the expression and function of muscarinic receptor in bone remain obscure In this study we investigated the expression and functional activities of muscarinic receptor subtypes in human osteoblast cell lines and animal and human bone tissue The mRNA levels of muscarinic receptor subtypes were detected by reverse-transcription polymerase chain reaction We found that muscarinic subtypes m1 m2 m3 m4 and m5 were expressed at different levels in human osteosarcoma HOS cells rat femur and human rib bone tissue m1 m4 m5 were in cultured mouse femur bone cells and cultured mouse calvarial bone cells m2 m3 m4 were in bovine bone The mRNA of neuronal markers light medium and heavy-neurofilament was not found in human bone tissues to exclude the possible contamination from neuronal tissue Methacholine induced an elevation in cytosolic calcium concentration and proliferation in HOS cells Both effects were blocked by atropine We conclude that muscarinic receptor is present in bone tissue to evoke calcium signaling and modulate cell proliferation Different muscarinic receptor subtypes are distributed in various parts of the animal skeletal system including the different s

• 出版日期2011-1-10
• 单位苏州大学