A novel neuropilin-1-binding sequence in the human T-cell lymphotropic virus type 1 envelope glycoprotein

作者：Kusunoki Hideki; Tanaka Toshiyuki; Kohno Toshiyuki; Matsuhashi Kazuhiko; Hosoda Kazuo; Wakamatsu Kaori; Hamaguchi Isao

来源：Biochimica et Biophysica Acta-Proteins and Proteomics, 2018, 1866(4): 541-548.

DOI：10.1016/j.bbapap.2018.02.003

• C-end rule/CendR
• HTLV-1
• Neuropilin-1
• NMR
• Surface unit/SU

Entry of human T-cell lymphotropic virus type 1 (HTLV-1) into host cells is mainly mediated by interactions between the viral envelope glycoprotein surface unit (SU) and three host receptors: glucose transporter type 1, heparin/heparan sulfate proteoglycan, and neuropilin-1 (Nrp1). Here, we analyzed the interaction between HTLV-1 SU and Nrp1 using nuclear magnetic resonance and isothermal titration calorimetry. We found that two SU peptides, residues 85-94 and residues 304-312, bound directly to the Nrp1 b1 domain with affinities of 7.4 and 17.7 mu M, respectively. The binding modes of both peptides were almost identical to those observed for Tuftsin and vascular endothelial growth factor A binding to the Nrp1 b1 domain. These results suggest that the C-terminal region of HTLV-1 SU contains a novel site for direct binding of virus to the Nrp1 b1 domain. Our biophysical characterization of the SU peptides may help in developing inhibitors of HTLV-1 entry.

• 出版日期2018-4