ISOLATION AND CHARACTERIZATION OF C-MYC, A CELLULAR HOMOLOG OF THE ONCOGENE (V-MYC) OF AVIAN MYELOCYTOMATOSIS VIRUS STRAIN-29

作者：VENNSTROM B; SHEINESS D; ZABIELSKI J; BISHOP JM

来源：Journal of Virology, 1982, 42(3): 773-779.

DOI：10.1128/jvi.42.3.773-779.1982

<jats:p>The chicken genome contains nucleotide sequences homologous to transforming genes (oncogenes) of a number of avian retroviruses. We have isolated chicken DNA (c-myc) that is homologous to the oncogene (v-myc) of the avian myelocytomatosis virus MC29 and have compared the structures of the cellular and viral genes. Results from restriction endonuclease mapping of c-myc and from analysis of heteroduplexes between the DNAs of the cellular and viral genes show that c-myc is homologous to 1,500 nucleotides in v-myc DNA. This homologous region is interrupted in c-myc by an intron-like sequence of 1,100 nucleotides which is absent from v-myc. Nuclear RNA from normal chicken cells contains at least five species of transcripts from c-myc ranging from 2.5 to 6.5 kilobases in length. By contrast, cytoplasm contains only the 2.5-kilobase c-myc RNA. These features of the c-myc gene and its nuclear transcripts are characteristic of normal cellular genes and suggest that the myc gene is of cellular rather than viral origin. The exons in c-myc may define two functional domains in the gene and may therefore facilitate the dissection of the different oncogenic potentials of the MC29 virus.</jats:p>

• 出版日期1982