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摘要
The hapten of the N-methylcarbamate insecticide metolcarb, 3-{[1-(3-(Methyl) phenyloxy) carbonyl] amino} propanoic acid (HOM), was synthesized and conjugated with the carrier proteins bovine serum albumin (BSA) and ovalbumin (OVA) respectively by the active ester method. The new zealand rabbits were immunized by the conjugate of HOM-BSA and the titres of anti-metolcarb serum (1.28x10(6)) were determined by a non-competitive indirect enzyme-linked immunosorbent assay (ELISA) procedure. The cross reaction indicate that the antiserum could specially recognize the insecticide metolcarb. After optimization of the ELISA conditions such as ionic strengths, organic solvents, pH values, blocking agents and so on, a competitive indirect ELISA procedure for the determination of metolcarb was established. Based on statistical analysis, the linear range of the procedure was from 1 mu g/L to 10(4) mu g/L, IC50 was 40.74 mu g/L, limit detection was 0.08 similar to 0.10 mu g/L, intra-assay velative standard deviatim (RSD) reached 2.9%, and inter-assay RSD reached 4.6%. The recoveries obtained by standard metolcarb addition to the different samples as rice, water and soil were 80%, 93.4% and 107% respectively. The produced polyclonal antibodies and the developed ELISA procedure may become a convenient and satisfied analytical toot for monitoring metolcarb residues in environmental and agricultural samples.
- 出版日期2006-2
- 单位南京农业大学
