Antimicrobial resistance of Listeria monocytogenes isolated from seafood and humans in Iran

作者：Abdollahzadeh Esmail; Ojagh Seyed Mahdi^*; Hosseini Hedayat; Ghaemi Ezzat Allah^*; Irajian Gholamreza; Heidarlo Masoud Naghizadeh

来源：Microbial Pathogenesis, 2016, 100: 70-74.

DOI：10.1016/j.micpath.2016.09.012

• Antimicrobial resistance
• Listeria monocytogenes
• Fish
• PFGE

Fourteen Listeria monocytogenes isolates previously collected from seafood (n = 7) and human patients (n = 7) were studied for their antimicrobial susceptibility against eight common antimicrobials (ampicillin, penicillin, gentamicin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, and cefotaxime). A high resistance level to ampicillin, cefotaxime (100%), and pencillin (57% in seafood isolates and 71.4% in clinical isolates) was observed in this study. However, all of the isolates were susceptible to trimethoprim-sulfamethoxazole, chloramphenicol, and tetracycline. Simultaneous resistance was identified in 4 clinical isolates (57.1%). Genotypic characterization of fish isolates (isolated from three fish species) was performed by pulsed-field gel electrophoresis (PFGE). A high diversity among fish isolates was observed. PFGE analyses distinguished the 4 isolates into 4 reproducible pulsotypes. There was no correlation between the antibiograms with pulsotypes. In conclusion, the resistance of seafood isolates to the antibiotics commonly used to treat listeriosis could be a potential health hazard for consumers.

• 出版日期2016-11