Modeling and MANOVA studies on toxicity and endocrine potential of packaging materials exposed to different extraction schemes

作者：Szczepanska Natalia; Kudlak Blazej^*; Tsakovski Stefan; Yotova Galina; Nedyalkova Miroslava; Simeonov Vasil; Dolega Anna; Namiesnik Jacek

来源：Environmental Research, 2018, 165: 294-305.

DOI：10.1016/j.envres.2018.05.004

• Toxicity
• Endocrine potential
• Packaging materials
• Extraction
• Experimental design
• Modeling
• MANOVA
• FTIR

The stability of the linings of packaging that is in contact with the goods stored has been of major concern during decades of the development of packaging materials. In this work, an attempt was undertaken to assess the applicability of using two bioassays (Microtox (R) and XenoScreen YES/YAS) in estimating the stability of packaging (cans, caps, multilayer material) and the impact of their degradation on the toxicity of some simulated media. The assessment of the impact of packaging storage conditions (temperature, disinfection, preservation, extracting and washing solvents) was planned and performed with i) regression modeling of the experimental effects on the ecotoxicity readings, ii) ANOVA and MANOVA estimation of the experimental conditions as significant factors affecting the toxicity results and iii) FTIR analysis of the packages. It is shown that the effects of temperature and extraction solvents could be quantitatively assessed by the agreement between all methods applied. It can be stated that temperature and acidity as well as the alcohol content in the sensitive media have the greatest impact on the toxicity of the extract and thus on the stability of the internal lining and the extractability of xenobiotics.

• 出版日期2018-8