Mediators of receptor tyrosine kinase activation in infantile fibrosarcoma: a Children%26apos;s Oncology Group study

作者：Gadd Samantha; Beezhold Patricia; Jennings Lawrence; George David; Leuer Katrin; Huang Chiang Ching; Huff Vicki; Tognon Cristina; Sorensen Poul H B; Triche Timothy; Coffin Cheryl M; Perlman Elizabeth J^*

来源：Journal of Pathology, 2012, 228(1): 119-130.

DOI：10.1002/path.4010

• mesoblastic nephroma
• infantile fibrosarcoma
• congenital fibrosarcoma
• GAB2
• Hippo
• ETV6-NTRK3

Infantile fibrosarcoma (IFS; also known as cellular congenital mesoblastic nephroma, CMN, when in the kidney) is a rare, undifferentiated tumour often characterized by the ETV6-NTRK3 fusion transcript. Our goal was to identify downstream pathways, diagnostic markers and potential therapeutic targets for IFS/CMN. Global gene expression, reverse-phase protein array and ETV6-NTRK3 fusion analyses were performed on 14 IFS/CMN and compared with 41 other paediatric renal tumours. These analyses confirm significant receptor tyrosine kinase (RTK) activation, with evidence of PI3-Akt, MAPK and SRC activation. In particular, GAB2 docking protein, STAT5-pTyr-694, STAT3-pSer-729 and YAP-pSer-127 were elevated, and TAZ-pSer-89 was decreased. This provides mRNA and proteomic evidence that GAB2, STAT activation and phosphorylation of the Hippo pathway transcription co-activators YAP and TAZ contribute to the RTK signal transduction in IFS/CMN. All IFS/CMN tumours displayed a distinctive gene expression pattern that may be diagnostically useful. Unexpectedly, abundant ETV6-NTRK3 transcript copies were present in only 7/14 IFS, with very low copy number in 3/14. An additional 4/14 were negative by RT-PCR and absence of ETV6-NTRK3 was confirmed by FISH for both ETV6 and NTRK3. Therefore, molecular mechanisms other than ETV6-NTRK3 fusion are responsible for the development of some IFS/CMNs and the absence of ETV6-NTRK3 fusion products should not exclude IFS/CMN as a diagnosis.

• 出版日期2012-9
• 单位西北大学