UVA-photoactivated riboflavin effect on isolated vaginal tissues derived from pelvic organ prolapse cases

作者：McMillan Kimberly S; Siddighi Sam; Hardesty Jeffrey S; Yune Joshua J; Chan Philip J^*

来源：International Urology and Nephrology, 2015, 47(1): 75-79.

DOI：10.1007/s11255-014-0836-5

• Pelvic organ prolapse
• Collagen
• Riboflavin
• UV cross-linking
• Tensile stiffness

To determine the optimal riboflavin exposure time before UVA irradiation and to study the effects of exogenous collagen on the mechano-tensile stiffness of isolated vaginal tissue strips after riboflavin UVA photoactivation.
Vaginal tissue strips from pelvic organ prolapse (POP) cases were soaked in 0.1 % riboflavin (0, 10, 20, 30 min), exposed to UVA photoactivation, and tensile stiffness was measured with a tensiometer. Collagen solution was injected (0.2 mL) into each strip, exposed to riboflavin with or without UVA photoactivation, and tensile stiffness was measured (n = 6).
Vaginal tissues treated with riboflavin for 10, 20 or 30 min followed by UVA irradiation displayed 21.2, 32.4 and 33.9 % stronger tensile stiffness, respectively. Exogenous collagen administered before riboflavin UVA photoactivation resulted in 20 % improvement in tensile stiffness. The tensile stiffness of vaginal tissues injected with collagen without the riboflavin UVA treatment was similar to control tissues.
The results demonstrated increased tensile stiffness in isolated POP-derived vaginal tissues after riboflavin UVA photoactivation suggesting improved mechanical properties from collagen cross-linking. Administering exogenous collagen before riboflavin UVA treatment also improved tensile stiffness. More studies are needed to corroborate the present minimally invasive approach for strengthening vaginal tissues.

• 出版日期2015-1