Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs

作者：Ahn Seong Joon; Hong Hye Kyoung; Na Young Mi; Park Sang Jun; Ahn Jeeyun; Oh Jaeseong; Chung Jae Yong; Park Kyu Hyung; Woo Se Joon^*

来源：Jove-Journal of Visualized Experiments, 2016, (113): e53878.

DOI：10.3791/53878

• Medicine
• Issue 113
• drug
• eye
• intraocular
• intravitreal
• pharmacokinetics
• rabbit

The intraocular route of drug administration enables the delivery of high concentrations of therapeutic drugs, while minimizing their systemic absorption. Several drugs are administered into the anterior chamber or vitreous, and the intraocular injection has been effective in curing various intraocular diseases. Rabbit eyes have been widely used for ophthalmic research, as the animal is easy to handle and economical compared to other mammals, and the size of a rabbit eye is similar to that of a human eye. Using a 30 G needle, drugs can be injected into the intracameral and intravitreal spaces of rabbit eyes. The eyeballs are then frozen until analysis, and can be divided into the aqueous humor, vitreous, and retina/choroid. The vitreous and retina/choroid samples can be homogenized and solubilized before analysis. Then, immunoassays can be performed to measure the concentrations of intraocular drugs in each compartment. Appropriate pharmacokinetic models can be used to calculate several parameters, such as the half-life and maximum concentration of the drug. Rabbit eyes can be a good model for pharmacokinetic studies of intraocular drugs.

• 出版日期2016-7