Antisense oligonucleotides targeting midkine inhibit tumor growth in an in situ human hepatocellular carcinoma model

作者：Dai Li cheng^*; Wang Xiang; Yao Xing; Min Li shan; Ping Jin liang; He Jian fang

来源：Acta Pharmacologica Sinica, 2007, 28(3): 453-458.

DOI：10.1111/j.1745-7254.2007.00532.x

• oligonucleotides
• antisense
• midkine
• carcinoma
• hepatocellular

Aim: To evaluate the in vivo antitumor effects of antisense oligonucleotides targeting midkine (MK-AS). Methods: An in situ human hepatocellular carcinoma (HCC) model was established in mice livers orthotopically. The MK-AS and 5-fluorouracil (5-Fu) were administered intravenously. The tumor sizes and plasma alpha-fetoprotein (AFP) were measured by calipers and radiation immunoassay respectively. The morphology of tumors was evaluated by hematoxylin-eosin staining of histological sections. Human MK, p53, Bax, Bcl-2, and caspase-3 protein content were detected by Western blotting. Results: MK-AS significantly inhibited in situ human HCC growth in mice compared with the saline group in a dose-dependent manner. After the treatment with MK-AS or with 5-Fu, the plasma AFP concentration decreased in a dose-dependent manner. Interestingly, MK-AS also clearly downregulated the protein level of Bcl-2, and upregulated p53, Bax, and caspase-3 in the hepatocellular carcinoma tissue. Conclusion: These results demonstrated that MK-AS was an effective antitumor antisense oligo-nucleotide in vivo in mice; its antitumor effect is associated with the increase of pro-apoptotic proteins, such as p53, Bax, and caspase-3, and the decrease of the anti-apoptotic protein, Bcl-2.

• 出版日期2007-3
• 单位湖州市中心医院