A new outlier removal approach for cDNA microarray normalization

作者：Wu, Yibo^*; Yang, Lirong; Liu, Hui; Sun, Hanchang; Xie, Hongwei

来源：Biotechniques, 2009, 47(2): 691-+.

DOI：10.2144/000113195

• outlier removal
• cDNA microarray
• normalization

Normalization is a critical step in the analysis of microarray gene expression data. For dual-labeled array, traditional normalization methods assume that the majority of genes are non-differentially expressed and that the number of overexpressed genes approximately equals the number of underexpressed genes. However, these assumptions are inappropriate in some particular conditions. Differentially expressed genes have a negative impact on normalization and are regarded as outliers in statistics. We propose a new outlier removal-based normalization method. Simulated and real data sets were analyzed, and our results demonstrate that our approach can significantly improve the precision of normalization by eliminating the impact of outliers, and efficiently identify candidates for differential expression.

• 出版日期2009-8
• 单位中国人民解放军国防科学技术大学; 中国人民解放军广州军区武汉总医院