Allogeneic bone graft devitalized in liquid nitrogen - sheep experimental study

作者：de Albuquerque Paulo Barros; Barni Brunna de Souza; Silva Renato Barbosa; Pinto Thadeu Mourao; Gianotti Giordano Cabral; da Luz Moura Lanucha Fidelis; Boss Mariana Zacouteguy; Alievi Marcelo Meller^*

来源：Ciencia Rural, 2015, 45(4): 718-723.

DOI：10.1590/0103-8478cr20140006

• allogeneic bone graft
• incorporation
• dynamic compressive plate
• orthopedics
• ovine

This study evaluated the allogeneic cortical bone graft incorporation after submission of the harvested fragment to a bout freezing in liquid nitrogen. Six adult sheep, clinically healthy, were submitted to a 7cm ostectomy of the tibial diaphysis. The fragment was submersed in a liquid nitrogen and implanted in another sheep missing a same-sized segment at the corresponding bone. Stabilization of the allograft in the host bone was accomplished by a dynamic compressive plate (DCP). Clinical and radiographic evaluations were performed in the immediate postoperatory period and in every 30 days for six months after surgery. The proximal and distal host-graft interfaces showed radiographic union at a mean post-operative time of 95 days in all the animals. The cortical bone allograft submitted to liquid nitrogen freezing provided adequate bone healing in the sheep model.

• 出版日期2015-4