Using global gene expression patterns to characterize Annexin V positive and negative human monocytes in culture

作者：Lund Per Kr^*; Ovstebo Reidun; Moller Anne Sophie W; Olstad Ole Kristoffer; Landsverk Kirsti S; Hellum Marit; Kierulf Peter

来源：Scandinavian Journal of Clinical & Laboratory Investigation, 2009, 69(2): 251-264.

DOI：10.1080/00365510802499399

• Apoptosis
• microarray
• monocytes
• macrophages
• sorting flow cytometry

Objective. To investigate the early apoptosis that may be detected by Annexin V binding to phosphatidylserine and propidium iodide (PI) exclusion in human monocytes. When studying monocytes in culture, less than 40 % of these cells survive after 7 days. Material and methods. In the first 4 h, 24 % of monocytes in culture develop into Annexin V+/PI- cells. Human monocytes were investigated at 0 h and sorted into Annexin V+ and Annexin V- by FACS after 4 h. Gene expression was examined by microarray analyses. Results. At 4 h, Annexin V+ monocytes versus Annexin V- cells showed 1220 differentially expressed genes. Ingenuity Pathway Analysis disclosed 153 genes related to cell death. Among these were caspase activators, caspase 6, Apaf 2 and FAS, as well as the autophagy gene ATG5. In addition, examination of the most up-regulated or down-regulated genes among the 1220 revealed genes involved in other biological processes, as well as genes not yet annotated. These included the non-annotated genes LOC28480 (fold change: 82) and 225767-at (fold change: 68) and the transcription factor SOX 4 (fold change: 24). Conclusions. We suggest that apoptosis in cultured monocytes, as evidenced by Annexin V+, operates through genes well known in apoptosis, but that the process also involves additional genes not commonly associated with apoptosis.

• 出版日期2009