Variability in Protein Expression in Marine-Derived Purpureocillium lilacinum Subjected to Salt and Chromium Stresses

作者：Lotlikar Nikita P; Damare Samir R^*

来源：Indian Journal of Microbiology, 2018, 58(3): 360-371.

DOI：10.1007/s12088-018-0733-1

• Filamentous fungi
• Glutathione
• Heat shock proteins
• LCMS QToF
• Proteomics
• Protein expression
• Salinity stress
• Stress tolerance mechanisms

Abiotic factors can cause substantial limitation of growth of microbes. A combination of salinity stress along with chromium (Cr 6), one of the carcinogen, can pose an immediate threat to any living system. To understand how salinity (0, 35 and 100 PSU) and Cr(VI) stress (0, 100 and 500 ppm), affects cells at the molecular level, the cellular response of Purpureocillium lilacinum to the individual as well the combination of both the stresses were studied by peptide mass fingerprinting technique. The study reports 1412 proteins, of which 105 proteins were found to be present across all conditions. The most prevalent functional class expressed was genetic information processing. Proteins involved in free radical scavenging were up-regulated in response to the oxidative stress generated due to both the applied stresses while expression of metal chelators, transporters systems, indicated towards multiple stress tolerance mechanisms to combat synergistic effects of salt and Cr stress.

• 出版日期2018-9