Diagnosis of soil-transmitted helminthiasis in an Amazonic community of Peru using multiple diagnostic techniques

作者：Machicado Jorge D^*; Marcos Luis A; Tello Raul; C****es Marco; Terashima Angelica; Gotuzzo Eduardo

来源：Transactions of the Royal Society of Tropical Medicine and Hygiene, 2012, 106(6): 333-339.

DOI：10.1016/j.trstmh.2012.03.004

• Soil-transmitted helminths
• Intestinal parasites
• Diagnosis
• Kato-Katz technique
• Spontaneous sedimentation in tube technique
• Peru

An observational descriptive study was conducted in a Shipibo-Conibo/Ese%26apos;Eja community of the rainforest in Peru to compare the Kato-Katz method and the spontaneous sedimentation in tube technique (SSTT) for the diagnosis of intestinal parasites as well as to report the prevalence of soil-transmitted helminth (STH) infections in this area. A total of 73 stool samples were collected and analysed by several parasitological techniques, including Kato-Katz, SS-TT, modified Baermann technique (MBT), agar plate culture, Harada-Mori culture and the direct smear examination. Kato-Katz and SSTT had the same rate of detection for Ascaris lumbricoides (5%), Trichuris trichiura (5%), hookworm (14%) and Hymenolepis nana (26%). The detection rate for Strongyloides stercoralis larvae was 16% by SSTT and 0% by Kato-Katz, but 18% by agar plate culture and 16% by MBT. The SSTT also had the advantage of detecting multiple intestinal protozoa such as Blastocystis hominis (40%), Giardia intestinalis (29%) and Entamoeba histolytica/E. dispar (16%). The most common intestinal parasites found in this community were B. hominis, G. intestinalis, H. nana, S. stercoralis and hookworm. In conclusion, the SSTT is not inferior to Kato-Katz for the diagnosis of common STH infections but is largely superior for detecting intestinal protozoa and S. stercoralis larvae.

• 出版日期2012-6
• 单位河北医科大学