Digestive enzymes characterization of krill (Euphausia superba) residues deoiled by supercritical carbon dioxide and organic solvents

作者：Ali Nehari Abdelkader; Kim Seon Bong; Lee Yang Bong; Chun Byung Soo^*

来源：Journal of Industrial and Engineering Chemistry, 2012, 18(4): 1314-1319.

DOI：10.1016/j.jiec.2012.01.026

• Krill
• Digestives enzymes
• Supercritical carbon dioxide extraction
• n-Hexane
• Acetone

In this study, Enzyme activities of krill were characterized before and after lipid extraction by supercritical carbon dioxide (SC-CO2) and organic solvent, n-hexane and acetone. Krill SC-CO2 extraction was performed under the conditions of temperature range from 35 to 45 degrees C and pressure, 150-250 bar for 2.5 h with a constant flow rate of 22 g/min. Extraction yields of lipids increased with pressure and temperature. The digestive enzyme activities of protease, lipase and amylase of SC-CO2 treated krill residues were slightly decreased comparing to organic solvent, n-hexane and acetone treated residues. In SC-CO2 treated samples, all of the digestive enzymes showed slightly higher temperature stability. In the other hand the crude extracts of SC-CO2 and n-hexane treated krill samples showed almost same optimum pH and pH stability for each of the digestive enzymes. It was also found in SDS-PAGE that there are no significant differences in protein patterns of the crude extracts of untreated, SC-CO2, n-hexane and acetone treated krill indicating no denaturation of proteins.

• 出版日期2012-7-25