科研之友
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摘要
The oligoglucose is taken as a model to establish a new method for the analysis of oligosaccharide structure by methylated, AcCl/HCI degradation, ( +) -2-methyl-2-,beta-naphthyl-1 3 -benzodioxole- 4-carboxylic acid ( ( +) -MNB carboxylic acid) fluorescent derivation and high performance liquid chromatography. The information of type monosaccharide contained, branch point of the oligosaccharide chain and the D,L-configuration of the monosaccharide could be obtained at the same time by this method. The operation is simple and the analytical sensitivity is 0. 2 pmol. The total operation period is within 5 h.
- 出版日期2005-3
- 单位复旦大学
