An effective method for the preparation of carrageenan oligosaccharides directly from Eucheuma cottonii using cellulase and recombinant kappa-carrageenase

作者：Duan, Fangyuan; Yu, Yuan; Liu, Zhemin; Tian, Lin; Mou, Haijin^*

来源：Algal Research-Biomass Biofuels and Bioproducts, 2016, 15: 93-99.

DOI：10.1016/j.algal.2016.02.006

• Carrageenan oligosaccharides
• Eucheuma cottonii
• Enzymatic hydrolysis
• Cellulase
• Carrageenase

Carrageenan oligosaccharides have drawn considerable attention for their potential application in various technologies. The objective of this study was to develop a high-efficiency method to prepare carrageenan oligosaccharides directly from Eucheuma cottonii using cellulase and recombinant.-carrageenase. The recombinant kappa-carrageenase was produced by Escherichia coli BL21-HTa-cgkZ, which expresses.-carrageenase from a marine bacterium. Dried seaweed was first digested with cellulase at pH 5.0 and 50 degrees C for 2 h, and then with recombinant.-carrageenase. To improve oligosaccharide recovery, the parameters of carrageenase hydrolysis were optimized using central composite design and validated. Using this method, E. cottonii oligosaccharides (ECO) were recovered with a 38% yield. High-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectrometry (FT-IR), and electrospray ionization-mass spectrometry (ESI-MS) results indicated that ECO possess characteristics specific to carrageenan oligosaccharides, in particular hexasaccharides.

• 出版日期2016-4
• 单位中国海洋大学