Depolarized Inactivation Overcomes Impaired Activation to Produce DRG Neuron Hyperexcitability in a Na(v)1.7 Mutation in a Patient with Distal Limb Pain

作者：Huang Jianying; Yang Yang; Dib Hajj Sulayman D; van Es Michael; Zhao Peng; Salomon Jody; Drenth Joost P H; Waxman Stephen G^*

来源：Journal of Neuroscience, 2014, 34(37): 12328-12340.

DOI：10.1523/JNEUROSCI.2773-14.2014

• DRG neurons
• mutation
• Na(v)1.7
• pain
• sodium channel

Sodium channel Na(v)1.7, encoded by SCN9A, is expressed in DRG neurons and regulates their excitability. Genetic and functional studies have established a critical contribution of Na(v)1.7 to human pain disorders. We have now characterized a novel Na(v)1.7 mutation (R1279P) from a female human subject with distal limb pain, in which depolarized fast inactivation overrides impaired activation to produce hyperexcitability and spontaneous firing in DRG neurons. Whole-cell voltage-clamp recordings in human embryonic kidney (HEK) 293 cells demonstrated that R1279P significantly depolarizes steady-state fast-, slow-, and closed-state inactivation. It accelerates deactivation, decelerates inactivation, and facilitates repriming. The mutation increases ramp currents in response to slow depolarizations. Our voltage-clamp analysis showed that R1279P depolarizes channel activation, a change that was supported by our multistate structural modeling. Because this mutation confers both gain-of-function and loss-of-function attributes on the Na(v)1.7 channel, we tested the impact of R1279P expression on DRG neuron excitability. Current-clamp studies reveal that R1279P depolarizes resting membrane potential, decreases current threshold, and increases firing frequency of evoked action potentials within small DRG neurons. The populations of spontaneously firing and repetitively firing neurons were increased by expressing R1279P. These observations indicate that the dominant proexcitatory gating changes associated with this mutation, including depolarized steady-state fast-, slow-, and closed-state inactivation, faster repriming, and larger ramp currents, override the depolarizing shift of activation, to produce hyperexcitability and spontaneous firing of nociceptive neurons that underlie pain.

• 出版日期2014-9-10